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Unless otherwise indicated, nucleotide sequences are written left to right in 5′ to 3′ orientation. I feel horrible. Nothing feels right about the situation. I really need some strong suggestion regarding this situation as what can I do to make things right .. The present invention also provides methods for administering a molecule that has been tested according to the above methods to a subject in need of treatment of a disease or condition. The term “therapeutic molecule” refers to any compound having a therapeutic effect in vivo for treatment of a disease or condition. In one embodiment, the toxic side effect includes neurotoxicity in vivo. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure is related. The present disclosure provides a method of testing or determining in vivo acute neurotoxicity of a molecule comprising measuring oscillations in intracellular free calcium concentration (“calcium oscillations”) in vitro in neuronal cells which are in contact with the molecule. EC50 is the median effective concentration of a therapeutic molecule relative to a vehicle or saline control. E2. The method of embodiment 1, wherein the calcium oscillations of the molecule are compared to the calcium oscillations in neuronal cells that are not exposed to the molecule (“control cells”)
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